Skip to content

Could possibly adding support to Seurat V5 #67

Description

@HarrisHan2k

Hi there,

I meet a "GetAssayData" error, when applying the progeny function to a Seurat object under Seurat V5 conditions.

Here is the error:

"Error:
! The slot argument of GetAssayData() was deprecated in SeuratObject 5.0.0 and is now defunct.
ℹ Please use the layer argument instead.
Traceback:

  1. progeny.Seurat(cdc2.object, scale = FALSE, organism = "Human",
    . top = 500, perm = 1, return_assay = TRUE)
  2. progeny(as.matrix(Seurat::GetAssayData(expr, slot = "data", assay = assay_name)),
    . scale = scale, organism = organism, top = top, perm = perm,
    . verbose = verbose, z_scores = z_scores, get_nulldist = get_nulldist,
    . assay_name = assay_name, return_assay = return_assay)
  3. as.matrix(Seurat::GetAssayData(expr, slot = "data", assay = assay_name))
  4. Seurat::GetAssayData(expr, slot = "data", assay = assay_name)
  5. GetAssayData.Seurat(expr, slot = "data", assay = assay_name)
  6. .Deprecate(when = "5.0.0", what = "GetAssayData(slot = )", with = "GetAssayData(layer = )")
  7. deprecate_stop(when = wv, what = what, with = with, env = caller,
    . ...)
  8. deprecate_stop0(msg)
  9. cnd_signal(error_cnd(c("lifecycle_error_deprecated", "defunctError"),
    . old = NULL, new = NULL, package = NULL, message = msg))
  10. signal_abort(cnd)
  11. signalCondition(cnd)"

Could you please help modify the codes to get the assay using another method, or adding a if-else sentence?

Activity

  1. nemke333 commented on Feb 2, 2026

    @nemke333

    I have the same issue would be great to get this patched!

  2. zhang163yv-pixel commented on Apr 14, 2026

    @zhang163yv-pixel

    use function :as.matrix

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment

Metadata

Metadata

Assignees

No one assigned

    Labels

    No labels
    No labels

    Type

    No type

    Projects

    No projects

      Milestone

      No milestone

      Relationships

      None yet

      Development

      No branches or pull requests

      Issue actions